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cse  (Proteintech)
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Figure 2. Expression <t>of</t> <t>CBS,</t> <t>CSE,</t> and 3-MST, as determined by immunostaining in placental villous tis- sue. (A) Representative CBS, CSE, and 3-MST expression, detected by immunostaining from normal and pre-eclamptic cases. In the normal group, both CBS and CSE were detected in the syncytiotrophoblast layer (double arrow). CBS, but not CSE, was also detected in the villous core fetal vessel endothelium (solid arrowhead). Expression of CBS and CSE were markedly reduced in syncytiotrophoblasts in pre-eclamptic cases (Figure 2A(b,d)) compared to controls. Expression of 3-MST was undetectable in placental specimens from both normal and pre-eclamptic cases (Figure 2A(e,f)). Bar = 50 µm. (B) Repre- sentative CBS, CSE, and 3-MST expression in the first, second, and third trimester placental specimens. Both CBS and CSE are strongly expressed in cyto- and syncytio-trophoblasts in the first, second, and third trimester placentas. CBS: a, b, and c; CSE: d, e, and f, respectively. Expression of 3-MST was detected in the first (g) and the second (h) trimester placentas, but not in the third/term (i) placentas. Bar = 50 µm.
Cse, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14787+1+ap/CBS+Antibody/10__3390_slash_pathophysiology32010010-32-61-66
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Figure 2. Expression of CBS, CSE, and 3-MST, as determined by immunostaining in placental villous tis- sue. (A) Representative CBS, CSE, and 3-MST expression, detected by immunostaining from normal and pre-eclamptic cases. In the normal group, both CBS and CSE were detected in the syncytiotrophoblast layer (double arrow). CBS, but not CSE, was also detected in the villous core fetal vessel endothelium (solid arrowhead). Expression of CBS and CSE were markedly reduced in syncytiotrophoblasts in pre-eclamptic cases (Figure 2A(b,d)) compared to controls. Expression of 3-MST was undetectable in placental specimens from both normal and pre-eclamptic cases (Figure 2A(e,f)). Bar = 50 µm. (B) Repre- sentative CBS, CSE, and 3-MST expression in the first, second, and third trimester placental specimens. Both CBS and CSE are strongly expressed in cyto- and syncytio-trophoblasts in the first, second, and third trimester placentas. CBS: a, b, and c; CSE: d, e, and f, respectively. Expression of 3-MST was detected in the first (g) and the second (h) trimester placentas, but not in the third/term (i) placentas. Bar = 50 µm.

Journal: Pathophysiology

Article Title: Altered Production and Cellular Levels of Hydrogen Sulfide (H2S) in Placental Trophoblasts from Pregnancies Affected by Pre-Eclampsia

doi: 10.3390/pathophysiology32010010

Figure Lengend Snippet: Figure 2. Expression of CBS, CSE, and 3-MST, as determined by immunostaining in placental villous tis- sue. (A) Representative CBS, CSE, and 3-MST expression, detected by immunostaining from normal and pre-eclamptic cases. In the normal group, both CBS and CSE were detected in the syncytiotrophoblast layer (double arrow). CBS, but not CSE, was also detected in the villous core fetal vessel endothelium (solid arrowhead). Expression of CBS and CSE were markedly reduced in syncytiotrophoblasts in pre-eclamptic cases (Figure 2A(b,d)) compared to controls. Expression of 3-MST was undetectable in placental specimens from both normal and pre-eclamptic cases (Figure 2A(e,f)). Bar = 50 µm. (B) Repre- sentative CBS, CSE, and 3-MST expression in the first, second, and third trimester placental specimens. Both CBS and CSE are strongly expressed in cyto- and syncytio-trophoblasts in the first, second, and third trimester placentas. CBS: a, b, and c; CSE: d, e, and f, respectively. Expression of 3-MST was detected in the first (g) and the second (h) trimester placentas, but not in the third/term (i) placentas. Bar = 50 µm.

Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM, D5523), Percoll (P1644), Hanks balanced salt solution (HBSS, H4891), Monobrombimane (MBB, B4380), and antibody against β-actin (A2066) were purchased from Sigma-Aldrich (St. Louis, MO, USA); Trypsin (LS003703) and Deoxyribonuclease (LS002139) were purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA); fetal bovine serum (FBS) was from Atlantic Biologicals (Flowery Branch, GA, USA); Antibodies against CBS (12217-1-AP) and CSE (14787-1-AP) were purchased from Proteintech (Rosemont, IL, USA); and antibody against 3-MST (AB224043) was from Abcam (Waltham, MA, USA).

Techniques: Expressing, Immunostaining

Figure 3. Trophoblast CBS and CSE protein expression by Western blot. Consistent with immunos- taining results, the levels of CBS and CSE protein expression were also significantly reduced in trophoblasts from pre-eclamptic (PE, n = 6) compared to those from normal (n = 7) cases. The bar graphs show relative protein expression of CBS and CSE after normalization with β-actin expression for each sample, * p < 0.05 and ** p < 0.01: PE vs. normal, respectively.

Journal: Pathophysiology

Article Title: Altered Production and Cellular Levels of Hydrogen Sulfide (H2S) in Placental Trophoblasts from Pregnancies Affected by Pre-Eclampsia

doi: 10.3390/pathophysiology32010010

Figure Lengend Snippet: Figure 3. Trophoblast CBS and CSE protein expression by Western blot. Consistent with immunos- taining results, the levels of CBS and CSE protein expression were also significantly reduced in trophoblasts from pre-eclamptic (PE, n = 6) compared to those from normal (n = 7) cases. The bar graphs show relative protein expression of CBS and CSE after normalization with β-actin expression for each sample, * p < 0.05 and ** p < 0.01: PE vs. normal, respectively.

Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM, D5523), Percoll (P1644), Hanks balanced salt solution (HBSS, H4891), Monobrombimane (MBB, B4380), and antibody against β-actin (A2066) were purchased from Sigma-Aldrich (St. Louis, MO, USA); Trypsin (LS003703) and Deoxyribonuclease (LS002139) were purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA); fetal bovine serum (FBS) was from Atlantic Biologicals (Flowery Branch, GA, USA); Antibodies against CBS (12217-1-AP) and CSE (14787-1-AP) were purchased from Proteintech (Rosemont, IL, USA); and antibody against 3-MST (AB224043) was from Abcam (Waltham, MA, USA).

Techniques: Expressing, Western Blot